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Device does not exists.
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Although the world has changed immensely since the release of Final Cut Pro on MacOS 9, and later. Â .The development of the third-generation T-cell epitope-vaccine strategy for HIV-1 Gag, aimed at inducing protective CTL responses in the absence of CD4+ T-cell help.
Immunization with Tat-based subunit vaccines induces antibody responses; however, induction of CTL requires peptide presentation and T-cell help. We have used a phosphatidylinositol (PI)-linked Tat-based subunit vaccine candidate to analyze the nature and size of immunodominant CTL epitopes and to assess their function and dependence on helper T-cell help. The CTL epitopes in HIV-1 Gag-derived sequences, most of which have not been identified in previous studies, are shown to be of shorter size and to conform to a T-cell receptor-defined class-I pattern. To provide T-cell help, we showed that Th1-devoted and bifunctional helper CD4+ T-cell clones secreted IL-2 and IL-4. Most importantly, T helper function was shown to be specifically required for the induction of CTL activity; whereas IL-2 and IL-4 had no effect on the induction of CTL. Further, peptide-pulsed cells were shown to induce CTL in the presence of neutralizing antibodies, indicating that this strategy is not limited to peptide delivery systems. This is a first demonstration of a minimum size of a CTL epitope and of the nature and extent of CTL help provided by helper CD4+ T-cells. The information obtained from this approach is important for the development of immunogens that will induce CTL in the absence of T helper cells.Master’s in Finance (MFin)
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